Essay --

Myocardial infarction is defined as pathological myocardial cell death due to a prolonged interruption of the business supply to the fondness, leading to a permanent loss of cardiomyocytes (Kristian Thygesen et al., 2012). The impact of myocardial infarction brought about a cascade of events followed by brand ecesis conferring protection to the insulted heart from being ruptured due to high pressure. Although it offers cardiac protection, scar tissues argon instead acellular and lack the normal biochemical properties of cardiac cell, thus enhancing the possibilities of disrupting the contractile function of the heart. These then may eventually leads to the down in the mouth left ventricular (LV) systolic and diastolic function of the injured heart (Joggerst & Hatzopoulos, 2009). Heart, has been considered as a terminally differentiated organ with an almost move out self-regenerative capacity back in the early days. The dogma was then distressed by the findings documented on th e presence of a small flock of a clonogenic endogenous cardiac waist cell pooling within the heart. These meet of endogenous cardiac still hunt cell demonstrates self-renewal capacity and multi-lineage differentiation authorisation suggesting that heart possess own intrinsic repair mechanism (Antonio P. Beltrami et al., 2003 Bearzi et al., 2007 Ellison, Nadal-Ginard, & Torella, 2012 Koudstaal et al., 2013). just the number of the endogenous cardiac stem cell is too low, adaptation the self-repair mechanism to fail (Beltrami et al., 2001). Stem cell based therapy holds promise in participating in the myocardial regeneration replacing the lost operable cardiomyocyte in the damaged myocardium. The roles of bone marrow stem cell graft in myocardial therapy too, have long... ... outcome of cardiac therapy. The main nous behind this novel therapeutic approach is the possibility to constrain the limitations of the face of stem cells in stem cell-based therapy. Taking the advant age of the conditioned fair and the benefit of paracrine mansion factors in promoting endogenous cardiac repair mechanism, we are trying to look at the best culture conditioned in terms of cell seeding density, glucose concentration, serum replacement and incubation time in order to generate conditioned medium that employs a potent paracrine signaling action that might enhance the ex vivo expansion of cardiac stem cell and potentially to be marketed as an off-the-shelf cardiac stem cell culture medium in the near future. Yet, more feat should be considered carefully before this novel noninvasive idea is apply in a more complex clinical setting.